Secondary Structure of Histones and DNA in Chromatin

Laser Raman spectroscopy indicates that the inner histones which are bound to DNA in chromatin or in isolated nu bodies are similar in conformation to the inner histones which are dissociated from DNA in high-salt solutions. This structure contains, on the average, 51 ± 5 percent α-helix and no subs...

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Veröffentlicht in:	Science (American Association for the Advancement of Science) 1977-07, Vol.197 (4301), p.385-388
Hauptverfasser:	Thomas, G. J., Prescott, B., Olins, D. E.
Format:	Artikel
Sprache:	eng
Schlagworte:	550200 - Biochemistry Amides Amino acids Animals BASIC BIOLOGICAL SCIENCES Chickens CHROMATIN Chromatin - ultrastructure Circular Dichroism DNA DNA - metabolism HISTONES Histones - metabolism LASERS Malaria Nucleic Acid Conformation NUCLEIC ACIDS ORGANIC COMPOUNDS Osmolar Concentration Parasites Protein Binding Protein Conformation PROTEINS Raman scattering RAMAN SPECTRA SPECTRA SPECTROSCOPY Spectrum Analysis, Raman STRUCTURAL CHEMICAL ANALYSIS Temperature Vaccination
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Beschreibung
Zusammenfassung:	Laser Raman spectroscopy indicates that the inner histones which are bound to DNA in chromatin or in isolated nu bodies are similar in conformation to the inner histones which are dissociated from DNA in high-salt solutions. This structure contains, on the average, 51 ± 5 percent α-helix and no substantial β-sheet conformation. It is proposed that the protein core of the nu body has a high α-helix content.
ISSN:	0036-8075 1095-9203
DOI:	10.1126/science.560060