Rat Insulin Genes: Construction of Plasmids Containing the Coding Sequences
Recombinant bacterial plasmids have been constructed that contain complementary DNA prepared from rat islets of Langerhans messenger RNA. Three plasmids contain cloned sequences representing the complete coding region of rat proinsulin I, part of the preproinsulin I prepeptide, and the untranslated...
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| Veröffentlicht in: | Science (American Association for the Advancement of Science) 1977-06, Vol.196 (4296), p.1313-1319 |
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| container_end_page | 1319 |
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| container_issue | 4296 |
| container_start_page | 1313 |
| container_title | Science (American Association for the Advancement of Science) |
| container_volume | 196 |
| creator | Ullrich, Axel Shine, John Chirgwin, John Pictet, Raymond Tischer, Edmund Rutter, William J. Goodman, Howard M. |
| description | Recombinant bacterial plasmids have been constructed that contain complementary DNA prepared from rat islets of Langerhans messenger RNA. Three plasmids contain cloned sequences representing the complete coding region of rat proinsulin I, part of the preproinsulin I prepeptide, and the untranslated 3′ terminal region of the mRNA. A fourth plasmid contains sequences derived from the A chain region of rat preproinsulin II. |
| doi_str_mv | 10.1126/science.325648 |
| format | Article |
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Three plasmids contain cloned sequences representing the complete coding region of rat proinsulin I, part of the preproinsulin I prepeptide, and the untranslated 3′ terminal region of the mRNA. A fourth plasmid contains sequences derived from the A chain region of rat preproinsulin II.</description><identifier>ISSN: 0036-8075</identifier><identifier>EISSN: 1095-9203</identifier><identifier>DOI: 10.1126/science.325648</identifier><identifier>PMID: 325648</identifier><language>eng</language><publisher>United States: American Association for the Advancement of Science</publisher><subject>Amino acids ; Animals ; Base Sequence ; Codon ; Complementary DNA ; DNA ; DNA - isolation & purification ; DNA Restriction Enzymes - metabolism ; DNA, Bacterial - analysis ; DNA, Bacterial - metabolism ; DNA, Recombinant - analysis ; DNA, Recombinant - metabolism ; Escherichia coli ; Extrachromosomal Inheritance ; Gels ; Genes ; Insulin ; Islets of Langerhans - analysis ; Ligation ; Messenger RNA ; Molecules ; Nucleotides ; Plasmids ; Proinsulin - biosynthesis ; Protein Precursors - biosynthesis ; Rats ; RNA, Messenger - isolation & purification ; Transformation, Genetic</subject><ispartof>Science (American Association for the Advancement of Science), 1977-06, Vol.196 (4296), p.1313-1319</ispartof><rights>Copyright 1977 American Association for the Advancement of Science</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c407t-a48600b4ef1b73474774cf1203f2bc513c7d086993577778e0310af6b2c49a733</citedby><cites>FETCH-LOGICAL-c407t-a48600b4ef1b73474774cf1203f2bc513c7d086993577778e0310af6b2c49a733</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/1744742$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/1744742$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>314,780,784,803,2882,2883,27923,27924,58016,58249</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/325648$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ullrich, Axel</creatorcontrib><creatorcontrib>Shine, John</creatorcontrib><creatorcontrib>Chirgwin, John</creatorcontrib><creatorcontrib>Pictet, Raymond</creatorcontrib><creatorcontrib>Tischer, Edmund</creatorcontrib><creatorcontrib>Rutter, William J.</creatorcontrib><creatorcontrib>Goodman, Howard M.</creatorcontrib><title>Rat Insulin Genes: Construction of Plasmids Containing the Coding Sequences</title><title>Science (American Association for the Advancement of Science)</title><addtitle>Science</addtitle><description>Recombinant bacterial plasmids have been constructed that contain complementary DNA prepared from rat islets of Langerhans messenger RNA. Three plasmids contain cloned sequences representing the complete coding region of rat proinsulin I, part of the preproinsulin I prepeptide, and the untranslated 3′ terminal region of the mRNA. A fourth plasmid contains sequences derived from the A chain region of rat preproinsulin II.</description><subject>Amino acids</subject><subject>Animals</subject><subject>Base Sequence</subject><subject>Codon</subject><subject>Complementary DNA</subject><subject>DNA</subject><subject>DNA - isolation & purification</subject><subject>DNA Restriction Enzymes - metabolism</subject><subject>DNA, Bacterial - analysis</subject><subject>DNA, Bacterial - metabolism</subject><subject>DNA, Recombinant - analysis</subject><subject>DNA, Recombinant - metabolism</subject><subject>Escherichia coli</subject><subject>Extrachromosomal Inheritance</subject><subject>Gels</subject><subject>Genes</subject><subject>Insulin</subject><subject>Islets of Langerhans - analysis</subject><subject>Ligation</subject><subject>Messenger RNA</subject><subject>Molecules</subject><subject>Nucleotides</subject><subject>Plasmids</subject><subject>Proinsulin - biosynthesis</subject><subject>Protein Precursors - biosynthesis</subject><subject>Rats</subject><subject>RNA, Messenger - isolation & purification</subject><subject>Transformation, Genetic</subject><issn>0036-8075</issn><issn>1095-9203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1977</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpdUD1PxDAMjRBfx8HKxNCJrYfTpE3Lhio4TpwE4mOu0jSFnNr0iNOBf09LTyDhxbbe87P9CDmnsKA0Sq5QGW2VXrAoTni6R2YUsjjMImD7ZAbAkjAFER-TE8QNwIBl7IgcTuwZeXiWPlhZ7Btjg6W2Gq-DvLPoXa-86WzQ1cFTI7E1FY6Al8Ya-x74Dz201Vi-6M9-vABPyUEtG9Rnuzwnb3e3r_l9uH5crvKbdag4CB9KniYAJdc1LQXjggvBVU2Hg-uoVDFlSlSQJlnGYjFEqoFRkHVSRopnUjA2J5eT7tZ1w2r0RWtQ6aaRVnc9FinLWAQ_xMVEVK5DdLouts600n0VFIrRvGJnXjHZMQxc7JT7stXVL_0_vEHfuT8xwYcvIvYNqoZz4A</recordid><startdate>19770617</startdate><enddate>19770617</enddate><creator>Ullrich, Axel</creator><creator>Shine, John</creator><creator>Chirgwin, John</creator><creator>Pictet, Raymond</creator><creator>Tischer, Edmund</creator><creator>Rutter, William J.</creator><creator>Goodman, Howard M.</creator><general>American Association for the Advancement of Science</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19770617</creationdate><title>Rat Insulin Genes: Construction of Plasmids Containing the Coding Sequences</title><author>Ullrich, Axel ; Shine, John ; Chirgwin, John ; Pictet, Raymond ; Tischer, Edmund ; Rutter, William J. ; Goodman, Howard M.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c407t-a48600b4ef1b73474774cf1203f2bc513c7d086993577778e0310af6b2c49a733</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1977</creationdate><topic>Amino acids</topic><topic>Animals</topic><topic>Base Sequence</topic><topic>Codon</topic><topic>Complementary DNA</topic><topic>DNA</topic><topic>DNA - isolation & purification</topic><topic>DNA Restriction Enzymes - metabolism</topic><topic>DNA, Bacterial - analysis</topic><topic>DNA, Bacterial - metabolism</topic><topic>DNA, Recombinant - analysis</topic><topic>DNA, Recombinant - metabolism</topic><topic>Escherichia coli</topic><topic>Extrachromosomal Inheritance</topic><topic>Gels</topic><topic>Genes</topic><topic>Insulin</topic><topic>Islets of Langerhans - analysis</topic><topic>Ligation</topic><topic>Messenger RNA</topic><topic>Molecules</topic><topic>Nucleotides</topic><topic>Plasmids</topic><topic>Proinsulin - biosynthesis</topic><topic>Protein Precursors - biosynthesis</topic><topic>Rats</topic><topic>RNA, Messenger - isolation & purification</topic><topic>Transformation, Genetic</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ullrich, Axel</creatorcontrib><creatorcontrib>Shine, John</creatorcontrib><creatorcontrib>Chirgwin, John</creatorcontrib><creatorcontrib>Pictet, Raymond</creatorcontrib><creatorcontrib>Tischer, Edmund</creatorcontrib><creatorcontrib>Rutter, William J.</creatorcontrib><creatorcontrib>Goodman, Howard M.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Science (American Association for the Advancement of Science)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ullrich, Axel</au><au>Shine, John</au><au>Chirgwin, John</au><au>Pictet, Raymond</au><au>Tischer, Edmund</au><au>Rutter, William J.</au><au>Goodman, Howard M.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Rat Insulin Genes: Construction of Plasmids Containing the Coding Sequences</atitle><jtitle>Science (American Association for the Advancement of Science)</jtitle><addtitle>Science</addtitle><date>1977-06-17</date><risdate>1977</risdate><volume>196</volume><issue>4296</issue><spage>1313</spage><epage>1319</epage><pages>1313-1319</pages><issn>0036-8075</issn><eissn>1095-9203</eissn><abstract>Recombinant bacterial plasmids have been constructed that contain complementary DNA prepared from rat islets of Langerhans messenger RNA. Three plasmids contain cloned sequences representing the complete coding region of rat proinsulin I, part of the preproinsulin I prepeptide, and the untranslated 3′ terminal region of the mRNA. A fourth plasmid contains sequences derived from the A chain region of rat preproinsulin II.</abstract><cop>United States</cop><pub>American Association for the Advancement of Science</pub><pmid>325648</pmid><doi>10.1126/science.325648</doi><tpages>7</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0036-8075 |
| ispartof | Science (American Association for the Advancement of Science), 1977-06, Vol.196 (4296), p.1313-1319 |
| issn | 0036-8075 1095-9203 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_83932073 |
| source | MEDLINE; JSTOR Archive Collection A-Z Listing; American Association for the Advancement of Science |
| subjects | Amino acids Animals Base Sequence Codon Complementary DNA DNA DNA - isolation & purification DNA Restriction Enzymes - metabolism DNA, Bacterial - analysis DNA, Bacterial - metabolism DNA, Recombinant - analysis DNA, Recombinant - metabolism Escherichia coli Extrachromosomal Inheritance Gels Genes Insulin Islets of Langerhans - analysis Ligation Messenger RNA Molecules Nucleotides Plasmids Proinsulin - biosynthesis Protein Precursors - biosynthesis Rats RNA, Messenger - isolation & purification Transformation, Genetic |
| title | Rat Insulin Genes: Construction of Plasmids Containing the Coding Sequences |
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