Rat Insulin Genes: Construction of Plasmids Containing the Coding Sequences

Recombinant bacterial plasmids have been constructed that contain complementary DNA prepared from rat islets of Langerhans messenger RNA. Three plasmids contain cloned sequences representing the complete coding region of rat proinsulin I, part of the preproinsulin I prepeptide, and the untranslated...

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Veröffentlicht in:	Science (American Association for the Advancement of Science) 1977-06, Vol.196 (4296), p.1313-1319
Hauptverfasser:	Ullrich, Axel, Shine, John, Chirgwin, John, Pictet, Raymond, Tischer, Edmund, Rutter, William J., Goodman, Howard M.
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino acids Animals Base Sequence Codon Complementary DNA DNA DNA - isolation & purification DNA Restriction Enzymes - metabolism DNA, Bacterial - analysis DNA, Bacterial - metabolism DNA, Recombinant - analysis DNA, Recombinant - metabolism Escherichia coli Extrachromosomal Inheritance Gels Genes Insulin Islets of Langerhans - analysis Ligation Messenger RNA Molecules Nucleotides Plasmids Proinsulin - biosynthesis Protein Precursors - biosynthesis Rats RNA, Messenger - isolation & purification Transformation, Genetic
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Beschreibung
Zusammenfassung:	Recombinant bacterial plasmids have been constructed that contain complementary DNA prepared from rat islets of Langerhans messenger RNA. Three plasmids contain cloned sequences representing the complete coding region of rat proinsulin I, part of the preproinsulin I prepeptide, and the untranslated 3′ terminal region of the mRNA. A fourth plasmid contains sequences derived from the A chain region of rat preproinsulin II.
ISSN:	0036-8075 1095-9203
DOI:	10.1126/science.325648