Interaction of the hemin 2 and 4 substituents with apo horseradish peroxidase

Interaction of the hemin 2 and 4 substituents with apo horseradish peroxidase

2-formyl, 4-vinyl deuterohemin ( 1) and 2-vinyl, 4-formyl deuterohemin ( 2) substituted horseradish peroxidases have been prepared from apoperoxidase and the respective hemins. The two hemins bind at different rates to the apoprotein and the resultant substituted peroxidases possess different visibl...

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Veröffentlicht in:Biochemical and biophysical research communications 1978-02, Vol.80 (4), p.698-703
Hauptverfasser: DiNello, Robert K., Dolphin, David
Format: Artikel
Sprache:eng
Schlagworte:
Apoenzymes
Heme - analogs & derivatives
Hemin
Horseradish Peroxidase
Iron
Kinetics
Peroxidases
Protein Binding
Protein Conformation
Spectrophotometry
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Zusammenfassung:2-formyl, 4-vinyl deuterohemin ( 1) and 2-vinyl, 4-formyl deuterohemin ( 2) substituted horseradish peroxidases have been prepared from apoperoxidase and the respective hemins. The two hemins bind at different rates to the apoprotein and the resultant substituted peroxidases possess different visible spectra and activities. These results indicate that the hemin 2 and 4 substituents interact with apoperoxidase and are not exposed to the solvent.
ISSN:0006-291X
1090-2104
DOI:10.1016/0006-291X(78)91300-1