In vitro growth of imaginal disks from Drosophila melanogaster

Imaginal disks were cultured in a new medium in vitro. Optimal growth occurred when the medium was supplemented with insulin and a juvenile hormone analog and conditioned by larval fat body. The disks grow in vitro by normal cell division and maintain their capacity to differentiate into normal patt...

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Veröffentlicht in:	Science (American Association for the Advancement of Science) 1977-04, Vol.196 (4288), p.438-440
Hauptverfasser:	Davis, K.T, Shearn, A
Format:	Artikel
Sprache:	eng
Schlagworte:	Adipose Tissue - physiology Adults Animals arthropods Cell Differentiation Cell Division Cell growth Cellular differentiation Culture Media Culture Techniques Cultured cells Drosophila Drosophila melanogaster - anatomy & histology Drosophila melanogaster - growth & development entomology Extremities - cytology Extremities - growth & development Fat body Insect larvae insects Insulin Insulin - pharmacology Larva - growth & development Larvae Larval development Metamorphosis, Biological Wings, Animal - cytology Wings, Animal - growth & development
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Beschreibung
Zusammenfassung:	Imaginal disks were cultured in a new medium in vitro. Optimal growth occurred when the medium was supplemented with insulin and a juvenile hormone analog and conditioned by larval fat body. The disks grow in vitro by normal cell division and maintain their capacity to differentiate into normal patterns of adult cuticular structures.
ISSN:	0036-8075 1095-9203
DOI:	10.1126/science.403606