Species and tissue differences in the catabolism of S-adenosyl-L-homocysteine: A quantitative, chromatographic study

Species and tissue differences in the catabolism of S-adenosyl-L-homocysteine: A quantitative, chromatographic study

Using a rapid, quantitative high pressure liquid chromatographic (HPLC) procedure for the separation of S-adenosyl-L-homocysteine (AdoHcy) and its purinic metabolites in rat and mouse brain, kidney and liver, we found that uric acid is the principal catabolic product of AdoHcy metabolism in the live...

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Veröffentlicht in:Life sciences (1973) 1977-01, Vol.20 (2), p.375-383
Hauptverfasser: Schatz, R.A., Vunnam, Chouda Rani, Sellinger, O.Z.
Format: Artikel
Sprache:eng
Schlagworte:
Adenine - metabolism
Animals
Brain - metabolism
Chromatography, High Pressure Liquid
Homocysteine - analogs & derivatives
Hydrolases - metabolism
Hypoxanthines - metabolism
Inosine - metabolism
Kidney - metabolism
Liver - metabolism
Male
Mice
Rats
S-Adenosylhomocysteine - metabolism
Species Specificity
Uric Acid - metabolism
Xanthines - metabolism
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Zusammenfassung:Using a rapid, quantitative high pressure liquid chromatographic (HPLC) procedure for the separation of S-adenosyl-L-homocysteine (AdoHcy) and its purinic metabolites in rat and mouse brain, kidney and liver, we found that uric acid is the principal catabolic product of AdoHcy metabolism in the liver, but that none forms in brain. Rat kidney formed about 10 times as much uric acid and half as much hypoxanthine as did mouse kidney. The HPLC procedure has been adapted to assay AdoHcy hydrolase activity which was found to be lowest in the brain and highest in the liver.
ISSN:0024-3205
1879-0631
DOI:10.1016/0024-3205(77)90334-4