Development of Amphioplus abditus (Verrill) (Echinodermata: Ophiuroidea): I. Larval Biology
A fraction rich in secretory granules was prepared from the pyloric caeca of Asterias amurensis by sucrose density gradient centrifugation. The freshly prepared fraction exhibited no casein-hydrolyzing activity- but showed nine times as much specific activity as that of tissue homogenates after incu...
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Veröffentlicht in: | The Biological bulletin 1977-02, Vol.152 (1), p.51-63 |
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Format: | Artikel |
Sprache: | eng |
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Zusammenfassung: | A fraction rich in secretory granules was prepared from the pyloric caeca of Asterias amurensis by sucrose density gradient centrifugation. The freshly prepared fraction exhibited no casein-hydrolyzing activity- but showed nine times as much specific activity as that of tissue homogenates after incubation at 37 degrees C for thirty minutes. Electron microscopy showed that the secretory granules were membrane-bound granules measuring 0.5-290 mu in diameter and contained dense and/or light amorphous substances. |
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ISSN: | 0006-3185 1939-8697 |
DOI: | 10.2307/1540726 |