Molecular events in lymphocyte differentiation: Stimulation of nonhistone nuclear protein synthesis in rabbit peripheral blood lymphocytes by anti-immunoglobulin

Using a sensitive double-labelling technique, changes in protein synthesis were investigated in rabbit peripheral blood lymphocytes at early times following stimulation with heterologous anti-immunoglobulin serum (GARG) or concanavalin A (Con A). At four hours following stimulation with either GARG or Con A, a striking increase in the synthesis of a nonhistone nuclear protein of apparent molecular weight 30–40,000 was observed. These results suggest that lymphocyte activation by both anti-immunoglobulin and Con A includes as an early event synthesis of a nonhistone nuclear protein.