Activation of Adenyl Cyclase and Adenosine 3', 5'-Monophosphate Phosphodiesterase in Rat Brain Synaptosomes

When the 105000g supernatant from rat brain cerebral cortex was fractionated using chromatography on a Sephadex G-25 Superfine, similar low molecular factors activated adenyl cyclase and adenosine 3', 5'-monophosphate (cyclic AMP) phosphodiesterase in rat brain synaptosomes. Therefore a comparative study of the effects of these factors with those of sodium fluoride (NaF) or imidazole was carried out. These factors additively enhanced NaF stimulated adenyl cyclase and did activate the particulate (synaptosomal) cyclic AMP phosphodiesterase but not soluble enzyme. These results suggest that these factors act on a distinctly different site from that of NaF and imidazole.