Detection, Quantitation, and Characterization of the Major Internal Virion Antigen of the Bovine Leukemia Virus by Radioimmunoassay

Detection, Quantitation, and Characterization of the Major Internal Virion Antigen of the Bovine Leukemia Virus by Radioimmunoassay

The major internal polypeptide of the bovine leukemia virus (BLV) was purified to homogeneity with the use of gel filtration and affinity chromatography. Like previous results, the protein had a molecular weight of 25,000 daltons as determined by electrophoresis in polyacrylamide gels with sodium do...

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Veröffentlicht in:JNCI : Journal of the National Cancer Institute 1976-10, Vol.57 (4), p.875-882
Hauptverfasser: McDonald, Hugh C., Ferrer, Jorge F.
Format: Artikel
Sprache:eng
Schlagworte:
Animals
Antigens, Viral - analysis
Binding, Competitive
Cattle
Cell Line
Epitopes
Leukemia Virus, Bovine - immunology
Molecular Weight
Radioimmunoassay
Retroviridae - immunology
Viral Proteins - analysis
Viral Proteins - immunology
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Zusammenfassung:The major internal polypeptide of the bovine leukemia virus (BLV) was purified to homogeneity with the use of gel filtration and affinity chromatography. Like previous results, the protein had a molecular weight of 25,000 daltons as determined by electrophoresis in polyacrylamide gels with sodium dodecyl sulfate, More than 90% of the 125I-labeled protein was precipitated by bovine sera that reacted in immunofluorescence tests with acetone-fixed BLV-infected cells. In contrast, minimal precipitation (
ISSN:0027-8874
1460-2105
DOI:10.1093/jnci/57.4.875