A denaturation map of sea urchin ribosomal DNA

A denaturation map of sea urchin ribosomal DNA

The ribosomal RNA genes from the sea urchin Lytechinus variegatus have been studied with the electron microsocope using the technique of denaturation mapping. A repeating pattern of denatured regions was found with an average repeat length of 3.87 +/- 0.24 mum. This corresponds to a DNA sequence of...

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Veröffentlicht in:Chromosoma 1976-11, Vol.58 (3), p.247-253
Hauptverfasser: Wilson, F E, Blin, N, Stafford, D W
Format: Artikel
Sprache:eng
Schlagworte:
Animals
DNA
Microscopy, Electron
Nucleic Acid Conformation
Nucleic Acid Denaturation
RNA, Ribosomal - biosynthesis
Sea Urchins
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Zusammenfassung:The ribosomal RNA genes from the sea urchin Lytechinus variegatus have been studied with the electron microsocope using the technique of denaturation mapping. A repeating pattern of denatured regions was found with an average repeat length of 3.87 +/- 0.24 mum. This corresponds to a DNA sequence of approximately 12,000 base pairs with a molecular weight of 8 X 10(6) daltons.
ISSN:0009-5915
1432-0886
DOI:10.1007/BF00292090