A technique for determining E rosette levels by cytofluorographic analysis

A method is described for determining the percentage of rosettes formed by sheep erythrocytes and human peripheral lymphocytes (ER) using cytofluorographic analysis (CFGA). The technique utilizes acridine orange dye for differentially staining nucleus and cytoplasm of the lymphocytes to distinguish...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Journal of immunological methods 1976-01, Vol.12 (1), p.9-17
Hauptverfasser: Goldberg, Nelson H., Kenady, Daniel E., Super, Bernard S., Chretie, Paul B.
Format: Artikel
Sprache:eng
Schlagworte:
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
Beschreibung
Zusammenfassung:A method is described for determining the percentage of rosettes formed by sheep erythrocytes and human peripheral lymphocytes (ER) using cytofluorographic analysis (CFGA). The technique utilizes acridine orange dye for differentially staining nucleus and cytoplasm of the lymphocytes to distinguish them from erythroctes, and glutaraldehyde for fixation of the rosettes. This technique was compared with light microscope counting (LMC) of ER. CFGA gave similar results with better reproducibility than LMC, entailed less time for counting, and markedly reduced operator fatigue.
ISSN:0022-1759
1872-7905
DOI:10.1016/0022-1759(76)90091-0