Partial characterization of rat hepatoma cell-surface glycopeptides possessing concanavalin a receptor activity

Papain digestion of Novikoff or AS-30D rat hepatoma cells released glycopeptides from the cell surface. That portion of the glycopeptides accessible to Sephadex G-50 was digested with pronase and the component glycopeptides partially resolved by ion-exchange chromatography. Each tumor type yielded t...

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Veröffentlicht in:	Biochemical and biophysical research communications 1976-01, Vol.72 (1), p.81-88
Hauptverfasser:	Robinson, John M., Smith, David F., Davis, Edward M., Gilliam, Ellen B., Capetillo, Sylvia C., Walborg, Earl F.
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acids - analysis Animals Carcinoma, Hepatocellular - metabolism Cell Membrane - metabolism Glycopeptides - metabolism Hexosamines - analysis Hexoses - analysis Liver Neoplasms - metabolism Neoplasms, Experimental - metabolism Rats Receptors, Concanavalin A - metabolism Receptors, Drug - metabolism Sialic Acids - analysis
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Zusammenfassung:	Papain digestion of Novikoff or AS-30D rat hepatoma cells released glycopeptides from the cell surface. That portion of the glycopeptides accessible to Sephadex G-50 was digested with pronase and the component glycopeptides partially resolved by ion-exchange chromatography. Each tumor type yielded two well resolved sialoglycopeptide fractions which possessed concanavalin A receptor activity. The amino acid and saccharide composition of these low molecular weight (3,100 ± 300 daltons) sialoglycopeptides was determined.
ISSN:	0006-291X 1090-2104
DOI:	10.1016/0006-291X(76)90963-3