A simplified technique for obtaining purified peripheral lymphocytes from chickens

A simplified technique for obtaining purified peripheral lymphocytes from chickens

A simplified technique for isolating purified avian peripheral lymphocytes using a Ficoll-diatrizoate density is described. The procedure requires only 2 ml. of blood, can be completed in 5 min., and is suitable for the repeated testing of relatively large numbers of birds.

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Bibliographische Detailangaben
Veröffentlicht in:Poultry science 1976-09, Vol.55 (5), p.1972-1973
Hauptverfasser: Archambault, L P, Dunlop, W R, Cucchiara, F P, Collins, W M, Corbett, A C
Format: Artikel
Sprache:eng
Schlagworte:
Animals
Cell Separation
Centrifugation, Density Gradient
Chickens - blood
Lymphocytes
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Beschreibung
Zusammenfassung:A simplified technique for isolating purified avian peripheral lymphocytes using a Ficoll-diatrizoate density is described. The procedure requires only 2 ml. of blood, can be completed in 5 min., and is suitable for the repeated testing of relatively large numbers of birds.
ISSN:0032-5791
DOI:10.3382/ps.0551972