The length and distribution of accessible DNA regions in human chromatin in situ

The length and distribution of accessible DNA regions in human chromatin in situ

The structure of chromatin in intact nuclei has been investigated by studying the kinetics of DNA degradation with exogenous nucleases. We find that human chromatin in its native state consists of relatively inaccessible DNA regions alternating with accessible regions of essentially uniform length....

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Veröffentlicht in:Biochemical and biophysical research communications 1976, Vol.72 (1), p.373-380
Hauptverfasser: Rosenberg, B.H., Deutsch, J.F., Cavalieri, L.F.
Format: Artikel
Sprache:eng
Schlagworte:
Cell Line
Cell Nucleus - ultrastructure
Chromatin - ultrastructure
DNA - analysis
Humans
Mathematics
Micrococcal Nuclease
Molecular Weight
Nucleic Acid Conformation
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Zusammenfassung:The structure of chromatin in intact nuclei has been investigated by studying the kinetics of DNA degradation with exogenous nucleases. We find that human chromatin in its native state consists of relatively inaccessible DNA regions alternating with accessible regions of essentially uniform length. The length of the accessible regions, as calculated from two independent sets of data, is about 50 base pairs.
ISSN:0006-291X
1090-2104
DOI:10.1016/0006-291X(76)91004-4