A technique for isolating turkey respiratory adenoviruses

Several turkey respiratory adenoviruses were isolated in turkey embryonic liver cells from nasal turbinate filtrate collected from clinically ill turkey poults during postmortem examination. Suspect adenovirus inoculum had to remain in cell culture for 5 to 7 days, and 9 blind passages were required...

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Veröffentlicht in:	Avian diseases 1976-10, Vol.20 (4), p.669-675
Hauptverfasser:	Simmons, Donald G., Gray, J. G.
Format:	Artikel
Sprache:	eng
Schlagworte:	Adenoviridae - isolation & purification Adenoviridae Infections - microbiology Adenoviridae Infections - veterinary Adenoviruses Animals Aviadenovirus - growth & development Aviadenovirus - immunology Aviadenovirus - isolation & purification Cell culture techniques Cells, Cultured Cytopathogenic Effect, Viral Embryonic cells Embryos Kidney cells Liver cells Poultry Diseases - microbiology Poults Respiratory diseases Respiratory Tract Infections - microbiology Respiratory Tract Infections - veterinary Turkeys Viruses
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Beschreibung
Zusammenfassung:	Several turkey respiratory adenoviruses were isolated in turkey embryonic liver cells from nasal turbinate filtrate collected from clinically ill turkey poults during postmortem examination. Suspect adenovirus inoculum had to remain in cell culture for 5 to 7 days, and 9 blind passages were required before a sample was declared negative. Once isolated, the turkey adenoviruses adapted rapidly to turkey kidney cells.
ISSN:	0005-2086 1938-4351
DOI:	10.2307/1589447