An automated procedure for the determination of phosphorus and alkaline phosphatase (bodansky) in serum

An automated modification of the Bodansky method for alkaline phosphatase and phosphorus in serum is presented. The tests can be performed at a rate of 40 tests/hour. Statistical evaluation showed that the methods are accurate and reproducible. Highest activity was obtained if a substrate adjusted to pH 9.60 (at 27°) or 9.40 (at 38°) was used. The resultant pH of buffered substrate and serum is between 9.00 and 9.10 (at 27°) and between pH 8.80 and 8.90 (at 38°). The normal values were found to be 1.3–4.0 units (7.0–21.5 International units) with a mean of 2.5 ± 0.59 S.D. An alternative procedure is listed which has an increased sensitivity and which eliminates contamination by specimens high in alkaline phosphatase activity but which can be run only at a speed of 20 tests/hour.