The structure of phosphatidyl glycerol prepared by phospholipase D-catalyzed transphosphatidylation from egg lecithin and glycerol

The structure of phosphatidyl glycerol prepared by phospholipase D-catalyzed transphosphatidylation from egg lecithin and glycerol

Phosphatidyl glycerol obtained by transphosphatidylation with phospholipase D (EC 3.1.4.4) from egg phosphatidyl choline and glycerol was hydrolyzed with phospholipase C (EC 3.1.4.3) and the configuration of the released α-glycerophosphate was determined by 3- sn-glycero-phosphate dehydrogenase (EC...

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Veröffentlicht in:Chemistry and physics of lipids 1976-01, Vol.17 (2), p.264-266
Hauptverfasser: Joutti, Anneli, Renkonen, Ossi
Format: Artikel
Sprache:eng
Schlagworte:
Bacillus cereus - enzymology
Egg Yolk
Female
Glycerol
Phosphatidylcholines
Phosphatidylglycerols - biosynthesis
Phospholipases
poultry products
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Zusammenfassung:Phosphatidyl glycerol obtained by transphosphatidylation with phospholipase D (EC 3.1.4.4) from egg phosphatidyl choline and glycerol was hydrolyzed with phospholipase C (EC 3.1.4.3) and the configuration of the released α-glycerophosphate was determined by 3- sn-glycero-phosphate dehydrogenase (EC 1.1.1.8). The phosphatidyl glycerol proved to be a racemic mixture of 3- sn-phosphatidyl-1′- sn-glycerol and 3- sn-phosphatidyl-3′- sn-glycerol.
ISSN:0009-3084
1873-2941
DOI:10.1016/0009-3084(76)90071-2