Procedure for enzymatic synthesis and isolation of radioactive long chain acyl-CoA esters

Procedure for enzymatic synthesis and isolation of radioactive long chain acyl-CoA esters

A method is described that yields pure long chain fatty acyl-CoA esters. It utilizes a partially purified long chain acyl-CoA synthetase that can be obtained from various sources. Product isolation involves butanol extraction and thin-layer chromatography. It is applicable to a wide range of fatty a...

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Veröffentlicht in:Analytical biochemistry 1976-05, Vol.73 (1), p.1-8
Hauptverfasser: Banis, Robert J., Roberts, Cynthia S., Stokes, Gilbert B., Tove, Samuel B.
Format: Artikel
Sprache:eng
Schlagworte:
Animals
Carbon Radioisotopes
Coenzyme A - metabolism
Coenzyme A Ligases - metabolism
Esters - biosynthesis
Fatty Acids - metabolism
Isotope Labeling
Liver - enzymology
Methods
Rats
Tritium
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Zusammenfassung:A method is described that yields pure long chain fatty acyl-CoA esters. It utilizes a partially purified long chain acyl-CoA synthetase that can be obtained from various sources. Product isolation involves butanol extraction and thin-layer chromatography. It is applicable to a wide range of fatty acids and is particularly suitable in preparing radioactive acyl-CoA esters.
ISSN:0003-2697
1096-0309
DOI:10.1016/0003-2697(76)90135-4