A Human Cold Agglutinin Which Binds Lacto-N-NeoTetraose

A human cold agglutinin (McC) has been isolated from the serum of a patient with Waldenström's macroglobulinemia by affinity chromatography with a column of glutaraldehyde-fixed erythrocyte stroma mixed with celite. The agglutinin, which is an IgM K, reacts weakly with normal adult erythrocytes...

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Veröffentlicht in:	The Journal of immunology (1950) 1976-09, Vol.117 (3), p.717-721
Hauptverfasser:	Tsai, Chao-Ming, Zopf, David A, Wistar, Richard, Jr, Ginsburg, Victor
Format:	Artikel
Sprache:	eng
Schlagworte:	ABO Blood-Group System Agglutinins - isolation & purification Antibody Specificity Autoantibodies - isolation & purification Cold Temperature Fetal Blood - immunology Galactosides Humans Immunoglobulin M - isolation & purification Male Oligosaccharides - immunology Waldenstrom Macroglobulinemia - immunology
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Beschreibung
Zusammenfassung:	A human cold agglutinin (McC) has been isolated from the serum of a patient with Waldenström's macroglobulinemia by affinity chromatography with a column of glutaraldehyde-fixed erythrocyte stroma mixed with celite. The agglutinin, which is an IgM K, reacts weakly with normal adult erythrocytes and strongly with cord cells as well as with adult Oh and Oi cells. The reactivity of all cell types tested was increased to approximately the same level by treatment of the cells with ficin. Inhibition studies indicate the agglutinin is specific for the nonreducing terminal carbohydrate sequence Gal(beta)1-4GlcNAc(beta)1-3Gal.
ISSN:	0022-1767 1550-6606