Rabbit corneal epithelial cells grown in vitro without serum

Primary outgrowth cultures of normal rabbit corneal epithelium can be initiated and propagated in vitro up to 6 days in serum-free medium. By the eighth day the majority of cells have ceased to divide. Epithelial cells grown without serum show DNA synthetic activity at a level comparable to control...

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Veröffentlicht in:Investigative ophthalmology 1976-08, Vol.15 (8), p.666-668
Hauptverfasser: Allman, MI, Harper, RA, Yanoff, M, Curfman, LJ, Cameron, JD, Flaxman, BA
Format: Artikel
Sprache:eng
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Zusammenfassung:Primary outgrowth cultures of normal rabbit corneal epithelium can be initiated and propagated in vitro up to 6 days in serum-free medium. By the eighth day the majority of cells have ceased to divide. Epithelial cells grown without serum show DNA synthetic activity at a level comparable to control cultures grown with added serum.
ISSN:0146-0404
0020-9988
1552-5783