Modification of the Shandon Southern apparatus MK II for SDS preparative polyacrylamide gel electrophoresis

The Shandon Southern preparative polyacrylamide gel electrophoresis apparatus was modified to permit use with SDS (sodium dodecyl sulfate) containing systems. Improvements include the elimination of the troublesome lower gel and modification of the elution plate to prevent localized high current densities and band overlapping. Separations were performed on the two subunits of Electrophorus electroplax Na + + K +-ATPase, the erythrocyte membrane protein Spectrin, and the trypic fragments of rabbit white skeletal muscle Ca 2+ + Mg 2+-ATPase.