Interruptions in the polynucleotide strands in bacteriophage DNA

Denatured phage DNA preparations are heterogeneous in the analytical ultra-centrifuge. Preparative zone sedimentation confirmed that the sedimentation diagrams reflect a true polydispersity of sedimentation coefficients among the polynucleotide strands. It is concluded that many phage particles—but...

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Veröffentlicht in:	Journal of molecular biology 1964, Vol.8 (1), p.1-10
Hauptverfasser:	Davison, P.F., Freifelder, D., Holloway, B.W.
Format:	Artikel
Sprache:	eng
Schlagworte:	Bacillus cereus Bacillus megaterium Bacillus subtilis Bacteriophages Centrifugation Chemical Phenomena Chemistry Culture Media DNA DNA, Viral Old Medline Polynucleotides Pseudomonas aeruginosa Spectrum Analysis
Online-Zugang:	Volltext
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Zusammenfassung:	Denatured phage DNA preparations are heterogeneous in the analytical ultra-centrifuge. Preparative zone sedimentation confirmed that the sedimentation diagrams reflect a true polydispersity of sedimentation coefficients among the polynucleotide strands. It is concluded that many phage particles—but not all—have one or more interruptions in the polynucleotide sequences, and the number of interruptions is consistent in a population of any one phage.
ISSN:	0022-2836 1089-8638
DOI:	10.1016/S0022-2836(64)80142-X