Protein-mediated uptake of vitamin B12 by isolated mitochondria

Protein-mediated uptake of vitamin B12 by isolated mitochondria

Protein-mediated B12 uptake by isolated rat liver mitochondria has been shown to be enhanced by plasma transcobalamin (TC-II) but not by salivary R binder in vitro. The process is enhanced by calcium and depends on active mitochondrial respiration. Following uptake, cyanocobalamin is converted to ad...

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Veröffentlicht in:Blood 1976-06, Vol.47 (6), p.923-930
Hauptverfasser: Gams, R A, Ryel, E M, Ostroy, F
Format: Artikel
Sprache:eng
Schlagworte:
Animals
Cell Fractionation
Humans
In Vitro Techniques
Male
Mitochondria, Liver - metabolism
Protein Binding
Rats
Saliva - metabolism
Transcobalamins - metabolism
Vitamin B 12 - isolation & purification
Vitamin B 12 - metabolism
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Zusammenfassung:Protein-mediated B12 uptake by isolated rat liver mitochondria has been shown to be enhanced by plasma transcobalamin (TC-II) but not by salivary R binder in vitro. The process is enhanced by calcium and depends on active mitochondrial respiration. Following uptake, cyanocobalamin is converted to adenosyl and methylcobalamins and released from the mitochondria. TC-II appears to be required for both cellular and mitochondrial uptake of vitamin B12.
ISSN:0006-4971
1528-0020
DOI:10.1182/blood.v47.6.923.923