The heavy DNA satellite of yeast and its relationship to the ribosomal RNA genes

The heavy satellite DNA of Saccharomyces carlsbergensis has been resolved by preparative NaI density gradient centrifugation. The rRNA genes were found to have a different density than the heavy satellite DNA. The heavy satellite DNA was found to be organized in clusters of molecular weight 15 × 10...

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Veröffentlicht in:	Arch. Biochem. Biophys.; (United States) 1976-01, Vol.174 (1), p.187-191
Hauptverfasser:	Lusby, E.W., deKloet, S.R.
Format:	Artikel
Sprache:	eng
Schlagworte:	550201 - Biochemistry- Tracer Techniques ALKALI METAL COMPOUNDS BASIC BIOLOGICAL SCIENCES CARBON 14 COMPOUNDS CELL CONSTITUENTS CENTRIFUGATION DENSITY DNA DNA - isolation & purification DNA - metabolism FUNGI GENES HALIDES HALOGEN COMPOUNDS HYBRIDIZATION IODIDES IODINE COMPOUNDS ISOTOPE APPLICATIONS LABELLED COMPOUNDS MICROORGANISMS MOLECULAR WEIGHT Nucleic Acid Hybridization NUCLEIC ACIDS ORGANIC COMPOUNDS ORGANOIDS PHYSICAL PROPERTIES PLANTS RIBOSOMES RNA RNA, Ribosomal - biosynthesis RNA, Ribosomal - isolation & purification SACCHAROMYCES Saccharomyces - metabolism SEPARATION PROCESSES SODIUM COMPOUNDS STRUCTURAL CHEMICAL ANALYSIS TRACER TECHNIQUES Transcription, Genetic YEASTS
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Zusammenfassung:	The heavy satellite DNA of Saccharomyces carlsbergensis has been resolved by preparative NaI density gradient centrifugation. The rRNA genes were found to have a different density than the heavy satellite DNA. The heavy satellite DNA was found to be organized in clusters of molecular weight 15 × 10 6, while the majority of the rRNA genes were found to be organized in clusters of 90 × 10 6 or more. These results show that the rRNA genes are not located in the heavy satellite DNA.
ISSN:	0003-9861 1096-0384
DOI:	10.1016/0003-9861(76)90337-4