Production of large quantities of 32P-labeled RNA tumor virus nucleic acid

A perfusion device was used to automatically collect 32P-labeled Rous sarcoma virus every 2 hr over an 8-day harvest period. During this time, a total of 64 × 10 6 counts of 32P-labeled 70S RNA was collected, and electrophoretic analysis showed that intact 35S RNA was obtained throughout the harvest...

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Veröffentlicht in:	Analytical biochemistry 1976-02, Vol.70 (2), p.354-358
Hauptverfasser:	Smith, Ralph E., Quade, Kristina
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Avian Sarcoma Viruses Chick Embryo Perfusion - instrumentation Phosphorus Radioisotopes RNA, Viral - isolation & purification
Online-Zugang:	Volltext
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