Production of large quantities of 32P-labeled RNA tumor virus nucleic acid

Production of large quantities of 32P-labeled RNA tumor virus nucleic acid

A perfusion device was used to automatically collect 32P-labeled Rous sarcoma virus every 2 hr over an 8-day harvest period. During this time, a total of 64 × 10 6 counts of 32P-labeled 70S RNA was collected, and electrophoretic analysis showed that intact 35S RNA was obtained throughout the harvest...

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Veröffentlicht in:Analytical biochemistry 1976-02, Vol.70 (2), p.354-358
Hauptverfasser: Smith, Ralph E., Quade, Kristina
Format: Artikel
Sprache:eng
Schlagworte:
Animals
Avian Sarcoma Viruses
Chick Embryo
Perfusion - instrumentation
Phosphorus Radioisotopes
RNA, Viral - isolation & purification
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Zusammenfassung:A perfusion device was used to automatically collect 32P-labeled Rous sarcoma virus every 2 hr over an 8-day harvest period. During this time, a total of 64 × 10 6 counts of 32P-labeled 70S RNA was collected, and electrophoretic analysis showed that intact 35S RNA was obtained throughout the harvest period.
ISSN:0003-2697
1096-0309
DOI:10.1016/0003-2697(76)90456-5