Purification and Characterization of a Sperm-Coating Antigen from Human Seminal Plasma

Purification and Characterization of a Sperm-Coating Antigen from Human Seminal Plasma

A sperm-coating antigen has been purified from human seminal plasma by a combination of fractionation methods including ammonium sulfate precipitation, Sephadex G-100 gel filtration, and diethylaminoethyl cellulose ion-exchange chromatography. The purified antigen was demonstrated to be immunologica...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Fertility and sterility 1976-06, Vol.27 (6), p.702-712
Hauptverfasser: Li, Tien Shun, Macajoux, Claude E., Devanesan, Mona M., Pelosi, Marco A., Caterini, Herik, Kaminetzky, Harold A.
Format: Artikel
Sprache:eng
Schlagworte:
Antigens - analysis
Antigens - isolation & purification
Chemical Precipitation
Chromatography, DEAE-Cellulose
Chromatography, Gel
Glycoproteins - analysis
Humans
Immunoelectrophoresis
Male
Molecular Weight
Semen - immunology
Spermatozoa - immunology
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
Beschreibung
Zusammenfassung:A sperm-coating antigen has been purified from human seminal plasma by a combination of fractionation methods including ammonium sulfate precipitation, Sephadex G-100 gel filtration, and diethylaminoethyl cellulose ion-exchange chromatography. The purified antigen was demonstrated to be immunologically identical with the original sperm-coating antigen, not derived from blood serum but having immunologically cross-relating antigens in milk and saliva, and a glycoprotein with a molecular weight of approximately 33,000. The purpose of purifying this antigen was discussed.
ISSN:0015-0282
1556-5653
DOI:10.1016/S0015-0282(16)41903-5