Preparation of rat epididymal α- l-fucosidase free from other glycosidases: its action on root-cap slime from Zea mays L

Affinity chromatography has been used in a rapid method of purification of rat epididymal α- l-fucosidase free of other glycosidases. The enzyme is suitable for analytical purposes and it has been used to investigate aspects of the structure of the fucose-containing slime synthesised by the outer root-cap cells of maize; less than 17% of the radioactive l-fucose incorporated into the slime from l-fucose- l- t was released by the enzyme