The involvement of biotin in preventing the fatty liver and kidney syndrome in chicks

Three experiments were carried out to investigate the involvement of vitamins in the fatty liver and kidney syndrome. The compounds studied, singly and in combination, were thiamin, riboflavin, nicotinic acid, pyridoxine, pantothenic acid, biotin, folic acid, vitamin B12, ascorbic acid, choline and inositol and, of these, only biotin prevented the syndrome. The minimum levels of supplemental dietary biotin required to prevent mortality varied from 0.05 to 0.15 mg/kg, depending on the diet. These levels were higher than the amounts required for maximum live-weight.