Partial elimination of latent Epstein-Barr virus genomes from virus-producing cells by cyclohexamide

EBV-producing P3 HRI cells were converted to a nonproductive state by treatment with cyclohexamide, and the number of EBV genomes decreased from 350 to 7 genomes per cell. Clones isolated from soft agar showed variations in the number of viral genomes per cell, ranging from 1 to 20. IUDR induced vir...

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Veröffentlicht in:	Virology (New York, N.Y.) N.Y.), 1976-01, Vol.70 (1), p.164-170
Hauptverfasser:	Tanaka, Akiko, Nonoyama, Meihan, Hampar, Berge
Format:	Artikel
Sprache:	eng
Schlagworte:	Antigens, Viral - analysis Bromodeoxyuridine - pharmacology Burkitt Lymphoma - microbiology Cell Division Cell Line Clone Cells Cycloheximide - pharmacology DNA Replication - drug effects DNA, Viral - biosynthesis Herpesvirus 4, Human - growth & development Herpesvirus 4, Human - immunology Herpesvirus 4, Human - metabolism Humans Idoxuridine - pharmacology Nucleic Acid Hybridization Virus Replication - drug effects
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Zusammenfassung:	EBV-producing P3 HRI cells were converted to a nonproductive state by treatment with cyclohexamide, and the number of EBV genomes decreased from 350 to 7 genomes per cell. Clones isolated from soft agar showed variations in the number of viral genomes per cell, ranging from 1 to 20. IUDR induced viral antigens and produced infectious viruses in all the clones except one, clone #9 carrying a single copy of EBV genome per cell. Growth characteristics of the clones were the same regardless of the number of EBV genomes present. The role of cyclohexamide on elimination of viral genomes and the significance of isolation of a clone with only a single copy of EBV genome per cell are discussed.
ISSN:	0042-6822 1096-0341
DOI:	10.1016/0042-6822(76)90246-4