Comparison of plasma progesterone assay in women by competitive protein binding (CPB) and radioimmunoassay (RIA)

Plasma progesterone levels during the follicular and luteal phases were compared when measured simultaneously by competitive protein binding (using guinea pig sera as binding agent) and radioimmunoassay (antiserum against progesterone-11-α-succinyl bovine serum albumin in rabbits), the values obtained were significantly different within each technique, depending on whether previous thin-layer Chromatographie purification of the extracts was employed or not. No significant differences were noticed between CPB and RIA when the Chromatographie step was used, but when it was omitted, CPB was greater than RIA at the follicular but not at the luteal phase.