Method for analysis of the anomers of glucose
An analytical method for the analysis of anomers of glucose has been described. This method employs glucose oxidase to destroy β-glucose. Analysis of residual glucose is performed by addition of peroxidase and chromogen. The method is operable in blood serum. Conditions have been worked out which wi...
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| Veröffentlicht in: | Analytical biochemistry 1963-11, Vol.6 (5), p.461-467 |
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| container_title | Analytical biochemistry |
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| creator | Keston, Albert S. Brandt, Richard |
| description | An analytical method for the analysis of anomers of glucose has been described. This method employs glucose oxidase to destroy β-glucose. Analysis of residual glucose is performed by addition of peroxidase and chromogen. The method is operable in blood serum. Conditions have been worked out which will destroy the β form quantitatively and leave over 99% of the α form. Data was presented that indicated a much higher specificity of glucose oxidase for β-glucose than previously reported (probably absolute specificity for β). |
| doi_str_mv | 10.1016/0003-2697(63)90098-8 |
| format | Article |
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This method employs glucose oxidase to destroy β-glucose. Analysis of residual glucose is performed by addition of peroxidase and chromogen. The method is operable in blood serum. Conditions have been worked out which will destroy the β form quantitatively and leave over 99% of the α form. Data was presented that indicated a much higher specificity of glucose oxidase for β-glucose than previously reported (probably absolute specificity for β).</description><identifier>ISSN: 0003-2697</identifier><identifier>EISSN: 1096-0309</identifier><identifier>DOI: 10.1016/0003-2697(63)90098-8</identifier><identifier>PMID: 14077639</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Blood Chemical Analysis ; Blood Glucose ; Chemistry Techniques, Analytical ; Glucose ; Glucose Oxidase ; Humans ; Old Medline ; Oxidation-Reduction</subject><ispartof>Analytical biochemistry, 1963-11, Vol.6 (5), p.461-467</ispartof><rights>1963</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c424t-5d7d1e9931c11d9fb8d70b83916c246eaa1808a7f90d04ef565a8def94758b543</citedby><cites>FETCH-LOGICAL-c424t-5d7d1e9931c11d9fb8d70b83916c246eaa1808a7f90d04ef565a8def94758b543</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/0003269763900988$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/14077639$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Keston, Albert S.</creatorcontrib><creatorcontrib>Brandt, Richard</creatorcontrib><title>Method for analysis of the anomers of glucose</title><title>Analytical biochemistry</title><addtitle>Anal Biochem</addtitle><description>An analytical method for the analysis of anomers of glucose has been described. This method employs glucose oxidase to destroy β-glucose. Analysis of residual glucose is performed by addition of peroxidase and chromogen. The method is operable in blood serum. Conditions have been worked out which will destroy the β form quantitatively and leave over 99% of the α form. Data was presented that indicated a much higher specificity of glucose oxidase for β-glucose than previously reported (probably absolute specificity for β).</description><subject>Blood Chemical Analysis</subject><subject>Blood Glucose</subject><subject>Chemistry Techniques, Analytical</subject><subject>Glucose</subject><subject>Glucose Oxidase</subject><subject>Humans</subject><subject>Old Medline</subject><subject>Oxidation-Reduction</subject><issn>0003-2697</issn><issn>1096-0309</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1963</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kE1LxDAQhoMo7rr6D0R6Ej1UJ5s0HxdBFr9gxYueQ5pM3Uq70aQV9t_b_UBvnoaB532HeQg5pXBFgYprAGD5VGh5IdilBtAqV3tkTEGLHBjofTL-RUbkKKUPAEp5IQ7JiHKQUjA9JvkzdovgsyrEzC5ts0p1ykKVdQsc9tBi3KzvTe9CwmNyUNkm4cluTsjb_d3r7DGfvzw8zW7nueNT3uWFl56i1ow6Sr2uSuUllIppKtyUC7SWKlBWVho8cKwKUVjlsdJcFqosOJuQ823vZwxfPabOtHVy2DR2iaFPRjHGuWByAPkWdDGkFLEyn7FubVwZCmatyawdmLUDI5jZaBrSE3K26-_LFv1faOdlAG62AA5fftcYTXI1Lh36OqLrjA_1_xd-ACfadRE</recordid><startdate>196311</startdate><enddate>196311</enddate><creator>Keston, Albert S.</creator><creator>Brandt, Richard</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>196311</creationdate><title>Method for analysis of the anomers of glucose</title><author>Keston, Albert S. ; Brandt, Richard</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c424t-5d7d1e9931c11d9fb8d70b83916c246eaa1808a7f90d04ef565a8def94758b543</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1963</creationdate><topic>Blood Chemical Analysis</topic><topic>Blood Glucose</topic><topic>Chemistry Techniques, Analytical</topic><topic>Glucose</topic><topic>Glucose Oxidase</topic><topic>Humans</topic><topic>Old Medline</topic><topic>Oxidation-Reduction</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Keston, Albert S.</creatorcontrib><creatorcontrib>Brandt, Richard</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Analytical biochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Keston, Albert S.</au><au>Brandt, Richard</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Method for analysis of the anomers of glucose</atitle><jtitle>Analytical biochemistry</jtitle><addtitle>Anal Biochem</addtitle><date>1963-11</date><risdate>1963</risdate><volume>6</volume><issue>5</issue><spage>461</spage><epage>467</epage><pages>461-467</pages><issn>0003-2697</issn><eissn>1096-0309</eissn><abstract>An analytical method for the analysis of anomers of glucose has been described. This method employs glucose oxidase to destroy β-glucose. Analysis of residual glucose is performed by addition of peroxidase and chromogen. The method is operable in blood serum. Conditions have been worked out which will destroy the β form quantitatively and leave over 99% of the α form. Data was presented that indicated a much higher specificity of glucose oxidase for β-glucose than previously reported (probably absolute specificity for β).</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>14077639</pmid><doi>10.1016/0003-2697(63)90098-8</doi><tpages>7</tpages></addata></record> |
| fulltext | fulltext |
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| ispartof | Analytical biochemistry, 1963-11, Vol.6 (5), p.461-467 |
| issn | 0003-2697 1096-0309 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_83344637 |
| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Blood Chemical Analysis Blood Glucose Chemistry Techniques, Analytical Glucose Glucose Oxidase Humans Old Medline Oxidation-Reduction |
| title | Method for analysis of the anomers of glucose |
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