Method for analysis of the anomers of glucose

An analytical method for the analysis of anomers of glucose has been described. This method employs glucose oxidase to destroy β-glucose. Analysis of residual glucose is performed by addition of peroxidase and chromogen. The method is operable in blood serum. Conditions have been worked out which wi...

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Veröffentlicht in:Analytical biochemistry 1963-11, Vol.6 (5), p.461-467
Hauptverfasser: Keston, Albert S., Brandt, Richard
Format: Artikel
Sprache:eng
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Zusammenfassung:An analytical method for the analysis of anomers of glucose has been described. This method employs glucose oxidase to destroy β-glucose. Analysis of residual glucose is performed by addition of peroxidase and chromogen. The method is operable in blood serum. Conditions have been worked out which will destroy the β form quantitatively and leave over 99% of the α form. Data was presented that indicated a much higher specificity of glucose oxidase for β-glucose than previously reported (probably absolute specificity for β).
ISSN:0003-2697
1096-0309
DOI:10.1016/0003-2697(63)90098-8