Antigenic components of guinea-pig tissues

Rabbits were immunized with extracts of ocular and systemic tissues of guinea-pigs. The immune sera were tosted against a wide range of guinea-pig tissues, using the Ouchterlony teehnique, immunoclectrophoresis and the fluorescent antibody method. All the sera showed antibodies to other tissues in addition to those used for immunization. Many of the cross reactions demonstrated by agar diffusion were due to serum components in the tissue extracts. However, some cross reactions—notably with antilens serum—were not due to serum components. The fluorescent antibody technique again showed numerous cross reactions between ocular and other tissues even after absorption of the immune serum with normal guinea-pig serum.