Gluconeogenesis from glycerol at rest and during exercise in normal, diabetic, and methylprednisolone-treated dogs

Glucose turnover, glycerol turnover, and the rate of incorporation of glycerol carbon into glucose were measured with the tracer technique (primed constant rate infusion) using 2- 3H-glucose and 14C-glycerol, at rest and during exercise (treadmill run) in normal (N), alloxan-diabetic (D), and methylprednisolone treated diabetic (MPD) dogs. At rest only 2%–3% of the hepatic glucose output arose from glycerol. Exercise increased gluconeogenesis about ninefold in N dogs and about fourfold in D and MPD animals, yet