Proviral DNA of Moloney Leukemia Virus: Purification and Visualization

Closed-circular proviral DNA of Moloney leukemia virus has been purified from a 10$^{7}$ excess of cellular and mitochondrial DNA. The DNA can be visualized in the electron microscope and has the contour length of a molecule with a molecular weight of about 5.5 × 10$^{6}$. Electron microscopic obser...

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Veröffentlicht in:	Science (American Association for the Advancement of Science) 1976-02, Vol.191 (4227), p.569-571
Hauptverfasser:	Gianni, A. M., Hutton, J. R., Smotkin, D., Weinberg, R. A.
Format:	Artikel
Sprache:	eng
Schlagworte:	Centrifugation Circular DNA DNA DNA, Circular - isolation & purification DNA, Viral - isolation & purification Genomes Microscopy, Electron Mitochondrial DNA Molecular weight Molecules Moloney murine leukemia virus - analysis Moloney murine leukemia virus - growth & development Nucleic Acid Hybridization Ribonucleases RNA RNA, Viral Sudden infant death syndrome Viral DNA
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Beschreibung
Zusammenfassung:	Closed-circular proviral DNA of Moloney leukemia virus has been purified from a 10$^{7}$ excess of cellular and mitochondrial DNA. The DNA can be visualized in the electron microscope and has the contour length of a molecule with a molecular weight of about 5.5 × 10$^{6}$. Electron microscopic observation of a hybrid between viral RNA and this circular DNA confirms the viral origin of this molecule.
ISSN:	0036-8075 1095-9203
DOI:	10.1126/science.1251192