Chromatographic studies on plant viruses: I. The isolation of potato virus X by means of various systems of adsorption chromatography
This work was an attempt to find a successful method for isolating potato virus X which would distinguish clearly between diseased plants and healthy plants. Two methods were tried, but without success. The first was chromatography on calcium phosphate and the second was chromatography on DEAE-cellu...
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Veröffentlicht in: | Virology (New York, N.Y.) N.Y.), 1963-03, Vol.19 (3), p.316-321 |
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Sprache: | eng |
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Zusammenfassung: | This work was an attempt to find a successful method for isolating potato virus X which would distinguish clearly between diseased plants and healthy plants.
Two methods were tried, but without success. The first was chromatography on calcium phosphate and the second was chromatography on DEAE-cellulose. A third method was then developed which gave distinct results. It involved chromatography on cellulose using polyethylene glycol and NaCl as solvent.
Extracts of healthy tomato plants and those infected with potato virus X were clarified by low speed centrifugation. Potato virus X was separated from host components of these extracts by treatment with chloroform, precipitation at pH 4.5, dissolution at pH 7.0, adsorption on cellulose in a column from a solution containing 6% polyethylene glycol and 4.5% NaCl, and elution of the cellulose column with solutions containing stepwise decreasing concentrations of polyethylene glycol and NaCl. Most of the healthy plant components remaining after precipitation at pH 4.5 were eluted by solutions containing polyethylene glycol and NaCl at concentrations of 4.0 and 3.0%, or 3.5 and 2.6%, respectively. But the virus was eluted by solutions containing polyethylene glycol and NaCl at concentrations of 1 and 0.75%, or 0.5 and 0.37%, respectively. |
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ISSN: | 0042-6822 1096-0341 |
DOI: | 10.1016/0042-6822(63)90070-9 |