A Hog Cholera Virus-Fluorescent Antibody System. Its Potential Use in Study of Embryonic Infection

Summary A hog cholera virus-fluorescent antibody system has been developed using pathogen-free pigs as the source of globulins. Heterologous globulin was developed by hy-perimmunizing pathogen-free genetically small pigs with 3 strains of virulent hog cholera virus. Success of the system appeared to be based on use of pigs having minimum of non-specific globulins, complement for antigen-antibody union, and globulins which approach maximum levels of conjugation to fluorescein isothiocyanate. Use of this system in the study of viral infections of swine embryos and for rapid diagnosis of hog cholera are discussed.