A Chemically Defined Medium for Growth of Animal Cells in Suspension

A Chemically Defined Medium for Growth of Animal Cells in Suspension

Summary A chemically defined medium is described that supports the growth of L, HeLa, monkey kidney, rat spleen, guinea pig kidney, and cat kidney cells in continuous suspension culture. Cell populations of 2.0 × 106 per ml to 3.1 × 106 per ml were obtained. The complete medium contains 33 component...

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Veröffentlicht in:Experimental biology and medicine (Maywood, N.J.) N.J.), 1963-02, Vol.112 (2), p.340-344
Hauptverfasser: Nagle, Stanley C., Tribble, Henry R., Anderson, Raymond E., Gary, Norman D.
Format: Artikel
Sprache:eng
Schlagworte:
Animals
Culture Media
Humans
Old Medline
Physiological Phenomena
Research Design
Suspensions
Tissue Culture Techniques
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Zusammenfassung:Summary A chemically defined medium is described that supports the growth of L, HeLa, monkey kidney, rat spleen, guinea pig kidney, and cat kidney cells in continuous suspension culture. Cell populations of 2.0 × 106 per ml to 3.1 × 106 per ml were obtained. The complete medium contains 33 components plus antibiotics and phenol red. The requirement for each component was determined for growth of a cat kidney cell line.
ISSN:0037-9727
1535-3702
1535-3699
DOI:10.3181/00379727-112-28038