A universal gradient apparatus for multiple organic solvents: II. Fractionation and elution of serum lipids

Chromatography of serum lipids on a silicic acid column by gradient elution and with continuous detection is described. The gradient is formed in a new organic gradient device based on the principle of E. Peterson and H. A. Sober ((1959) Anal. Chem. 31, 857), starting from six initial organic solven...

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Veröffentlicht in:	Analytical biochemistry 1975-11, Vol.69 (1), p.29-33
Hauptverfasser:	Vandamme, D., Declercq, B., Denoo, A., Blaton, V., Peeters, H.
Format:	Artikel
Sprache:	eng
Schlagworte:	Chromatography - methods Chromatography, Thin Layer - methods Humans Lipids - blood Silicic Acid Solvents
Online-Zugang:	Volltext
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Beschreibung
Zusammenfassung:	Chromatography of serum lipids on a silicic acid column by gradient elution and with continuous detection is described. The gradient is formed in a new organic gradient device based on the principle of E. Peterson and H. A. Sober ((1959) Anal. Chem. 31, 857), starting from six initial organic solvents. The detection is realized by flame ionization on a LCM2 liquid chromatography detector (Pye-Unicam). The technique is less solvent- and time-consuming and is of interest in preparative separation and analysis of lipophilic substances.
ISSN:	0003-2697 1096-0309
DOI:	10.1016/0003-2697(75)90561-8