Biological assay of prostaglandin using rat uterus

Isometric contraction of rat uterus with PGF2α was more reliable and stable than when a stomach preparation was used. The method of PG bioassay using rat uterus was as follows : ovariectomized rats were given 10 μg of estrone s. c. 48 hr before the decapitation. Uterine strips were suspended in 2 ml...

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Veröffentlicht in:	Folia Pharmacologica Japonica 1975, Vol.71(7), pp.675-682
Hauptverfasser:	TAKEUCHI, Kumeji, YAMADA, Kenji, AIZAWA, Yoshio
Format:	Artikel
Sprache:	jpn
Schlagworte:	Animals Atropine - pharmacology Biological Assay - methods Estrone - pharmacology Female In Vitro Techniques Intestine, Small - analysis Male Muscle Contraction - drug effects Population Prostaglandins F - analysis Rats Stomach - drug effects Temperature Uterine Contraction - drug effects Uterus - drug effects
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Beschreibung
Zusammenfassung:	Isometric contraction of rat uterus with PGF2α was more reliable and stable than when a stomach preparation was used. The method of PG bioassay using rat uterus was as follows : ovariectomized rats were given 10 μg of estrone s. c. 48 hr before the decapitation. Uterine strips were suspended in 2 ml of organ bath containing modified Locke-Ringer solution at 25°C, and the isometric contraction was determined. As little as 4 ng/ml of PGF2α was determined quantitatively with this method.
ISSN:	0015-5691 1347-8397
DOI:	10.1254/fpj.71.675