Analysis of Macromolecule—Macromolecule Interactions by the Moving Boundary Method

Analysis of Macromolecule—Macromolecule Interactions by the Moving Boundary Method

A constituent velocity equation has been derived which permits analysis of macro-molecule (P)—macromolecule (A) interactions in which a number of complexes, PA, PA2, PA3, … PAn, are in rapid equilibrium. In previous treatments, it was assumed that the velocity of complex PA, (i = 1, 2, 3, …, n) incr...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Journal of biochemistry (Tokyo) 1975-07, Vol.78 (1), p.199-203
1. Verfasser: Janado, M
Format: Artikel
Sprache:eng
Schlagworte:
Electrophoresis
Ligands
Macromolecular Substances
Mathematics
Methods
Molecular Conformation
Molecular Weight
Protein Binding
Ultracentrifugation
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
Beschreibung
Zusammenfassung:A constituent velocity equation has been derived which permits analysis of macro-molecule (P)—macromolecule (A) interactions in which a number of complexes, PA, PA2, PA3, … PAn, are in rapid equilibrium. In previous treatments, it was assumed that the velocity of complex PA, (i = 1, 2, 3, …, n) increased linearly with i, an assumption limiting the validity of the resulting equation. In the present treatment, the velocity of PAi is expressed in the form of a polynomial function in i and a generalized equation has been formulated.
ISSN:0021-924X
1756-2651
1756-2651
DOI:10.1093/oxfordjournals.jbchem.a130884