A direct enzymic method for the determination of reduced glutathione in blood and other tissues

A direct enzymic method for the determination of reduced glutathione in blood and other tissues

A new, rapid and specific method for the determination of reduced glutathione has been developed. The method differs from enzymic methods described previously in that reduced glutathione is a substrate for the enzyme used in the assay. The assay is based upon the reaction C 6H 3(NO 2) 2Cl + GSH / C...

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Veröffentlicht in:Biochemical medicine 1975-07, Vol.13 (3), p.287-292
Hauptverfasser: Crowley, Charles, Gillham, Brian, Thorn, Michael B.
Format: Artikel
Sprache:eng
Schlagworte:
Adult
Animals
Glutathione - analysis
Glutathione - blood
Glutathione Transferase
Humans
Kidney - analysis
Liver - analysis
Oxidation-Reduction
Rats
Spectrophotometry, Ultraviolet - methods
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Zusammenfassung:A new, rapid and specific method for the determination of reduced glutathione has been developed. The method differs from enzymic methods described previously in that reduced glutathione is a substrate for the enzyme used in the assay. The assay is based upon the reaction C 6H 3(NO 2) 2Cl + GSH / C 6H 3(NO 2) 2SG + H + + Cl −, catalysed by glutathione S-transferases (e.g., EC 2.5.1.13) from rat liver. Other thiols found in tissues do not interfere in the assay. Results obtained by applying the present method to various tissues were consistently lower than those from the DTNB method.
ISSN:0006-2944
1557-7996
DOI:10.1016/0006-2944(75)90087-3