Studies on the heterogeneity of messenger RNA from E. Coli
The behaviour of messenger RNA from pulse-labelled Escherichia coli cells when sedimented on a linear eoneentration gradient of sucrose depends on the mode of extraction of the cells and on the concentration of Mg 2+ ions during the sedimentation. Extraction procedures, which lessen the degradative...
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Veröffentlicht in: | Journal of molecular biology 1962-09, Vol.5 (3), p.311-324 |
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creator | Monier, Roger Naono, Shiro Hayes, Donal Hayes, Françoise Gros, François |
description | The behaviour of messenger RNA from pulse-labelled
Escherichia coli cells when sedimented on a linear eoneentration gradient of sucrose depends on the mode of extraction of the cells and on the concentration of Mg
2+ ions during the sedimentation. Extraction procedures, which lessen the degradative effects of nucleolytic enzymes, give messenger RNA preparations richer in heavy fractions. When Mg
2+ ions are present during the centrifugation, messenger RNA partly associates with ribosomal RNA components and sediments with them.
Chromatography on methylated serum albumin-coated Kieselguhr shows that messenger RNA is heterogeneous. The chromatographic behaviour of messenger RNA fractions depends on their molecular size and probably also on their nucleotide composition. Some fractions of messenger RNA appear to have molecular sizes which correspond to sedimentation coefficients in the range 23 to 30 s, and which are probably able to control the synthesis of the longest protein monomers. The relative proportions of the various fractions are influenced by the extraction procedure but some of them are present in every preparation and probably pre-exist in growing cells. |
doi_str_mv | 10.1016/S0022-2836(62)80075-8 |
format | Article |
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Escherichia coli cells when sedimented on a linear eoneentration gradient of sucrose depends on the mode of extraction of the cells and on the concentration of Mg
2+ ions during the sedimentation. Extraction procedures, which lessen the degradative effects of nucleolytic enzymes, give messenger RNA preparations richer in heavy fractions. When Mg
2+ ions are present during the centrifugation, messenger RNA partly associates with ribosomal RNA components and sediments with them.
Chromatography on methylated serum albumin-coated Kieselguhr shows that messenger RNA is heterogeneous. The chromatographic behaviour of messenger RNA fractions depends on their molecular size and probably also on their nucleotide composition. Some fractions of messenger RNA appear to have molecular sizes which correspond to sedimentation coefficients in the range 23 to 30 s, and which are probably able to control the synthesis of the longest protein monomers. The relative proportions of the various fractions are influenced by the extraction procedure but some of them are present in every preparation and probably pre-exist in growing cells.</description><identifier>ISSN: 0022-2836</identifier><identifier>EISSN: 1089-8638</identifier><identifier>DOI: 10.1016/S0022-2836(62)80075-8</identifier><identifier>PMID: 13936044</identifier><language>eng</language><publisher>England: Elsevier Ltd</publisher><subject>Escherichia coli ; Old Medline ; RNA ; RNA, Bacterial ; RNA, Messenger</subject><ispartof>Journal of molecular biology, 1962-09, Vol.5 (3), p.311-324</ispartof><rights>1962 Academic Press Inc. (London) Ltd.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c410t-c1cd2973b6af2cf4b94603e46e5b19d23178c716e0d8d9268437b01aa651eaa83</citedby><cites>FETCH-LOGICAL-c410t-c1cd2973b6af2cf4b94603e46e5b19d23178c716e0d8d9268437b01aa651eaa83</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0022283662800758$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65534</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/13936044$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Monier, Roger</creatorcontrib><creatorcontrib>Naono, Shiro</creatorcontrib><creatorcontrib>Hayes, Donal</creatorcontrib><creatorcontrib>Hayes, Françoise</creatorcontrib><creatorcontrib>Gros, François</creatorcontrib><title>Studies on the heterogeneity of messenger RNA from E. Coli</title><title>Journal of molecular biology</title><addtitle>J Mol Biol</addtitle><description>The behaviour of messenger RNA from pulse-labelled
Escherichia coli cells when sedimented on a linear eoneentration gradient of sucrose depends on the mode of extraction of the cells and on the concentration of Mg
2+ ions during the sedimentation. Extraction procedures, which lessen the degradative effects of nucleolytic enzymes, give messenger RNA preparations richer in heavy fractions. When Mg
2+ ions are present during the centrifugation, messenger RNA partly associates with ribosomal RNA components and sediments with them.
Chromatography on methylated serum albumin-coated Kieselguhr shows that messenger RNA is heterogeneous. The chromatographic behaviour of messenger RNA fractions depends on their molecular size and probably also on their nucleotide composition. Some fractions of messenger RNA appear to have molecular sizes which correspond to sedimentation coefficients in the range 23 to 30 s, and which are probably able to control the synthesis of the longest protein monomers. The relative proportions of the various fractions are influenced by the extraction procedure but some of them are present in every preparation and probably pre-exist in growing cells.</description><subject>Escherichia coli</subject><subject>Old Medline</subject><subject>RNA</subject><subject>RNA, Bacterial</subject><subject>RNA, Messenger</subject><issn>0022-2836</issn><issn>1089-8638</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1962</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkEtLAzEQgIMoWh8_QclJ9LA6eWw260VK8QVFweo5ZLOzbaS70WQr-O9tbdGjp7l8Mx_zEXLM4IIBU5cTAM4zroU6U_xcAxR5prfIgIEuM62E3iaDX2SP7Kf0BgC5kHqX7DFRCgVSDsjVpF_UHhMNHe1nSGfYYwxT7ND3XzQ0tMWUsJtipM-PQ9rE0NKbCzoKc39Idho7T3i0mQfk9fbmZXSfjZ_uHkbDceYkgz5zzNW8LESlbMNdI6tSKhAoFeYVK2suWKFdwRRCreuSKy1FUQGzVuUMrdXigJyu777H8LHA1JvWJ4fzue0wLJLRAkpZSLUE8zXoYkgpYmPeo29t_DIMzCqa-YlmVkWM4uYnmlkJTjaCRdVi_be1qbQErtcALt_89BhNch47h7WP6HpTB_-P4hsFHXnj</recordid><startdate>196209</startdate><enddate>196209</enddate><creator>Monier, Roger</creator><creator>Naono, Shiro</creator><creator>Hayes, Donal</creator><creator>Hayes, Françoise</creator><creator>Gros, François</creator><general>Elsevier Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>196209</creationdate><title>Studies on the heterogeneity of messenger RNA from E. Coli</title><author>Monier, Roger ; Naono, Shiro ; Hayes, Donal ; Hayes, Françoise ; Gros, François</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c410t-c1cd2973b6af2cf4b94603e46e5b19d23178c716e0d8d9268437b01aa651eaa83</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1962</creationdate><topic>Escherichia coli</topic><topic>Old Medline</topic><topic>RNA</topic><topic>RNA, Bacterial</topic><topic>RNA, Messenger</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Monier, Roger</creatorcontrib><creatorcontrib>Naono, Shiro</creatorcontrib><creatorcontrib>Hayes, Donal</creatorcontrib><creatorcontrib>Hayes, Françoise</creatorcontrib><creatorcontrib>Gros, François</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of molecular biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Monier, Roger</au><au>Naono, Shiro</au><au>Hayes, Donal</au><au>Hayes, Françoise</au><au>Gros, François</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Studies on the heterogeneity of messenger RNA from E. Coli</atitle><jtitle>Journal of molecular biology</jtitle><addtitle>J Mol Biol</addtitle><date>1962-09</date><risdate>1962</risdate><volume>5</volume><issue>3</issue><spage>311</spage><epage>324</epage><pages>311-324</pages><issn>0022-2836</issn><eissn>1089-8638</eissn><abstract>The behaviour of messenger RNA from pulse-labelled
Escherichia coli cells when sedimented on a linear eoneentration gradient of sucrose depends on the mode of extraction of the cells and on the concentration of Mg
2+ ions during the sedimentation. Extraction procedures, which lessen the degradative effects of nucleolytic enzymes, give messenger RNA preparations richer in heavy fractions. When Mg
2+ ions are present during the centrifugation, messenger RNA partly associates with ribosomal RNA components and sediments with them.
Chromatography on methylated serum albumin-coated Kieselguhr shows that messenger RNA is heterogeneous. The chromatographic behaviour of messenger RNA fractions depends on their molecular size and probably also on their nucleotide composition. Some fractions of messenger RNA appear to have molecular sizes which correspond to sedimentation coefficients in the range 23 to 30 s, and which are probably able to control the synthesis of the longest protein monomers. The relative proportions of the various fractions are influenced by the extraction procedure but some of them are present in every preparation and probably pre-exist in growing cells.</abstract><cop>England</cop><pub>Elsevier Ltd</pub><pmid>13936044</pmid><doi>10.1016/S0022-2836(62)80075-8</doi><tpages>14</tpages><oa>free_for_read</oa></addata></record> |
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subjects | Escherichia coli Old Medline RNA RNA, Bacterial RNA, Messenger |
title | Studies on the heterogeneity of messenger RNA from E. Coli |
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