Radioactive Labeling of Proteins with an Iodinated Amidination Reagent

An iodinated phenolic imidoester has been synthesized for the labeling of proteins to high specific activities with radioactive iodine. The main advantage of this two-step labeling method is that it obviates direct contact of the protein with deleterious oxidizing agents, such as chloramine-T, press...

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Veröffentlicht in:	Journal of dental research 1975-10, Vol.54 (3_suppl), p.86-92
1. Verfasser:	Wood, Frederick T.
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Benzene Derivatives - chemical synthesis Dentistry Dogs Hydrogen-Ion Concentration Imides - chemical synthesis Indicators and Reagents Iodine Radioisotopes Isotope Labeling - methods Proteins Serum Albumin Spectrophotometry, Ultraviolet Temperature
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Beschreibung
Zusammenfassung:	An iodinated phenolic imidoester has been synthesized for the labeling of proteins to high specific activities with radioactive iodine. The main advantage of this two-step labeling method is that it obviates direct contact of the protein with deleterious oxidizing agents, such as chloramine-T, pressent in direct methods of iodinating proteins.
ISSN:	0022-0345 1544-0591
DOI:	10.1177/002203457505400310011