A human/mouse hybrid model for the study of human genetic factors influencing tumor cell growth

A human/mouse hybrid model for the study of human genetic factors influencing tumor cell growth

Somatic cell hybridization (fusion) was carried out between cells of the murine lymphoma L‐5178Y(r) and peripheral human lymphocytes. The L‐5178Y(r) line is deficient in hypoxanthine‐guanine phosphorybosyl transferase (HGPRT). Using a selective medium system, it was possible to isolate hybrid cell c...

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Veröffentlicht in:Journal of surgical oncology 1975, Vol.7 (4), p.323-327
Hauptverfasser: Koshman, Robert W., Koo, Jarley, Thurston, Olin G.
Format: Artikel
Sprache:eng
Schlagworte:
Animals
Cell Fusion
cell hybrids
Humans
Hybrid Cells
Karyotyping
Lymphocytes
Lymphoma
Mice
model systems
Models, Biological
Neoplasms - genetics
Sex Chromosomes
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Zusammenfassung:Somatic cell hybridization (fusion) was carried out between cells of the murine lymphoma L‐5178Y(r) and peripheral human lymphocytes. The L‐5178Y(r) line is deficient in hypoxanthine‐guanine phosphorybosyl transferase (HGPRT). Using a selective medium system, it was possible to isolate hybrid cell clones having the complete complement of murine chromosomes as well as the human X chromosome on which the gene for HGPRT is located. This model system may be used to study the effect of the human X chromosome on the phenotype of the murine lymphoma.
ISSN:0022-4790
1096-9098
DOI:10.1002/jso.2930070409