Agar gel chromatography of rat liver ribosomal ribonucleic acids

Ribosomal RNA (28 s rRNA) of rat liver is selectively retained in 2 or 4% squashed agar gels equilibrated at 24–25°C with a sodium dodecyl sulfate-Tris-EDTA buffer containing 0.7 m NaCl. The absorbed polynucleotide could be recovered by elution with 0.1 m NaCl in the same buffer. Agar-gel electropho...

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Veröffentlicht in:Analytical biochemistry 1975-08, Vol.67 (2), p.462-467
1. Verfasser: Popović, D.A.
Format: Artikel
Sprache:eng
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Zusammenfassung:Ribosomal RNA (28 s rRNA) of rat liver is selectively retained in 2 or 4% squashed agar gels equilibrated at 24–25°C with a sodium dodecyl sulfate-Tris-EDTA buffer containing 0.7 m NaCl. The absorbed polynucleotide could be recovered by elution with 0.1 m NaCl in the same buffer. Agar-gel electrophoresis and nucleotide composition indicate that the separation is close to quantitative. Column beds of 100–200 ml were used in the range of 3–6 mg of RNA. The procedure is simple, rapid and reproducible and gives excellent separation of 28 and 18 s rat liver rRNA.
ISSN:0003-2697
1096-0309
DOI:10.1016/0003-2697(75)90319-X