Large-scale preparation of Na,K-ATPase from ox brain

A method for the isolation of membrane-bound Na,K-ATPase in quantity from brain gray matter is described. The method permits a large amount of enzyme to be obtained rather quickly with about 60% of the original activity of Na,K-ATPase of the tissue being recovered. The enzyme is stable, it has a specific activity of about 200 μmoles ATP split per mg protein per hour. Mg-ATPase comprises about 1% of the total ATPase activity. The enzymatic properties of this Na,K-ATPase do not differ from those in the literature; the turnover number is about 9300 min −1.