An improved method for determination of uric acid in serum

A method is described for the determination of uric acid in serum which is sufficiently precise and reliable to follow small changes at low levels. The advantage of the use in the acetate form of the anion exchange resin Dowex 2-X8 is pointed out. The influence of protein and other anion components...

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Veröffentlicht in:Analytical biochemistry 1962-07, Vol.4 (1), p.24-27
Hauptverfasser: Carr, Marie H., Pressman, Berton C.
Format: Artikel
Sprache:eng
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Zusammenfassung:A method is described for the determination of uric acid in serum which is sufficiently precise and reliable to follow small changes at low levels. The advantage of the use in the acetate form of the anion exchange resin Dowex 2-X8 is pointed out. The influence of protein and other anion components of serum on the absorbance of uric acid and on its elution from the resin is noted. Normal values of serum uric acid in dogs as well as man, with sampling deviations, and reproducibility of the method at both levels is demonstrated.
ISSN:0003-2697
1096-0309
DOI:10.1016/0003-2697(62)90015-5